conditioned freezing chambers  (Med Associates Inc)

Journal: Frontiers in Genetics

Article Title: Affective phenotypes in heterozygous LRRK2 R1441G knock-in mice

doi: 10.3389/fgene.2025.1629897

Figure Lengend Snippet: Evidence for depression-related behavioural phenotypes in heterozygous KI +/− mice. (A) All mice showed a preference for the sucrose solution with the percentage of sucrose solution exceeding 50%, but a reduction was evident in the KI +/− group, whose preference was significantly lower than KI +/+ mice ( p < 0.05 *) and marginally below WT mice ( p = 0.06 # ) based on pairwise comparisons by Fisher’s LSD. (B) Latency to shuttle (left) and percentage of escape failures (right) in the conditioned active avoidance task illustrate the impact of prior exposure to inescapable shock versus escapable shock. This difference resulted in a significant main effect of pre-exposure experience (* p < 0.01). (C) Immobility time (as a percentage) across successive 1-min bins on days 1 and 2 are separately depicted, showing earlier and stronger immobility in KI+/− and KI+/+ mice than WT mice (especially on Day 2). (D) Mean immobility time (as a percentage) in the FST is shown for the full 10-min test (bins 1–10, left) and the last 6 min (bins 5–10, right). Both KI+/− and KI+/+ mice exhibited significantly higher immobility than WT mice in post hoc pairwise comparisons ( p < 0.05 *). The SPT and FST were conducted in mice from Cohort A. n = 12 (6♀+ 6♂) per genotype. The LH experiment included mice in both cohorts A and B. n = 12 (6♀+ 6♂) per genotype per pre-exposure condition. All data are presented as the mean ± SE. Overlaid scatter points represent data from individual mice (green dots = ♂; pink dots = ♀).

Article Snippet: Two conditioned freezing chambers (MED-VFC-USB-M, Med Associates, VT, United States) were used.

Techniques:

Journal: Frontiers in Genetics

Article Title: Affective phenotypes in heterozygous LRRK2 R1441G knock-in mice

doi: 10.3389/fgene.2025.1629897

Figure Lengend Snippet: Selected phenotypes in anxiety-related behaviour. (A) The distribution of time spent in the open arms of the EPM relative to time in all arms (in percent) provides a measure of anxiety-like behaviour. A significant difference was detected using Fisher’s LSD test between KI +/− and WT mice (*), while the difference between KI +/− and KI +/+ did not achieve significance (# at p = 0.09). The EPM test included mice in both cohort A and B. n = 24 (12♀+ 12♂) per genotype. Overlaid scatter points represent data from individual mice (green dots = ♂; pink dots = ♀). (B) The expression of conditioned fear to the tone-CS, assessed 48 h after tone–shock pairings, was indexed by percentage time freezing in the tone test. Tone-freezing was continuously tracked for 8 min after the initial 2-min pre-CS period. The data presented are averaged across three consecutive test days (separate plots for each day are presented in ). (C) The expression of foreground contextual fear conditioning was assessed by returning the animals to the shocked context “A” on the first and third days (1A and 3A) and the neutral context “B” on the second and fourth days (2B and 4B) after conditioning. Higher levels of freezing in context “A” relative context “B” supported the expression of context-specific conditioned fear response. For (B,C) , n = 12 (6♀+ 6♂) per genotype. All data are presented as the mean ± SE.

Article Snippet: Two conditioned freezing chambers (MED-VFC-USB-M, Med Associates, VT, United States) were used.

Techniques: Expressing

Journal: Journal of Neuroinflammation

Article Title: Reduced acute neuroinflammation and improved functional recovery after traumatic brain injury by α-linolenic acid supplementation in mice

doi: 10.1186/s12974-016-0714-4

Figure Lengend Snippet: Effect of low or adequate α-linolenic acid diet on motor and cognitive changes after TBI. a , b Impaired motor performance in the rotarod ( a ) and beam walk tests ( b ) due to TBI was recovered faster in mice on adequate α-linolenic acid (ALA) diet compared to those on the low ALA diet that had less brain DHA. c Mice on adequate ALA diet showed more freezing after contextual fear conditioning compared to the mice on low ALA diet for both sham and TBI mice. *** p < 0.001, ** p < 0.01, * p < 0.05 compared to the respective low ALA diet group. Data are expressed as mean ± SEM. n = 8–14

Article Snippet: Mice were individually placed inside the fear conditioning chamber (Freeze Monitor, San Diego Instruments, San Diego, CA, USA) on the first day and allowed to explore for 5 min. On the following day, mice were given two shocks at 120 and 150 s after being introduced into the fear conditioning chamber and were taken out of the chamber 60 s after the last shock.

Techniques: